Ion-Sensitive Field-Effect Transistor-Based Biosensor for PSA Antigen Concentration Measurement Using Microfluidic System

Document Type : Research Paper

Authors

Electrical and Computer Engineering Faculty, Semnan University, Semnan, Iran

Abstract

Prostate cancer is one of the most common cancers in men. Prostate-Specific Antigen (PSA) is an important biomarker in the diagnosis of prostate cancer. In the present paper, an Ion-Sensitive Field-Effect Transistor (ISFET) is introduced, employing microfluidic technology to detect PSA antigens efficiently. PSA antigen is our analyte in this sensor. Due to the PSA antigen's acidity and sensor sensitivity to the hydrogen ions and pH index, absorbing the hydrogen ion by the OH receptor on the sensor surface modulates the drive current and the device's threshold voltage. The electroosmotic flow is induced inside the microchannel by applying a voltage to the electrodes on the walls of the microchannel. Consequently, turbulence in the fluid flow in the channel has occurred that effectively moves the intended analytes toward the sensor surface. The biosensor performance is investigated by the simulations carried on in COMSOL. Our simulation results indicate that the proposed structure facilitates rapid detection and measurement of PSA concentration.

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[1] Majji, Sankararao, Chandra Sekhar Dash, and Asisa Kumar Panigrahy. "Ion Sensitive Field Effect Transistor as a Bio-compatible Device: A Review." 2022 International Conference on Electronics and Renewable Systems (ICEARS). IEEE, 2022.
[2] Poghossian, Arshak, and Michael J. Schöning. "Recent progress in silicon-based biologically sensitive field-effect devices." Current Opinion in Electrochemistry 29 (2021): 100811.
[3] Ma, Xiaohao, et al. "Recent advances in ion‐sensitive field‐effect transistors for biosensing applications." Electrochemical Science Advances (2022): e2100163.
[4] Wadhera, Tanu, et al. "Recent advances and progress in development of the field-effect transistor biosensor: A review." Journal of Electronic Materials 48.12 (2019): 7635-7646.
[5] Mehta, Aditya, et al. "Machine Learning Techniques for Performance Enhancement of Si 3 N 4-gate ISFET pH Sensor." 2020 IEEE 17th India Council International Conference (INDICON). IEEE, 2020.
[6] Wang, Hui, and Naiyun Tang. "A modified TCAD simulation model for a-InGaZnO based ISFETs on GaAs substrate for pH sensing applications." Materials Research Express 8.9 (2021): 095901.
[7] Acar, Gizem, et al. "An ISFET Sensor-Integrated Micromixer for pH Measurements." 2020 21st International Conference on Thermal, Mechanical and Multi-Physics Simulation and Experiments in Microelectronics and Microsystems (EuroSimE). IEEE, 2020.
[8] Cao, Shengli, et al. "ISFET‐based sensors for (bio) chemical applications: A review." Electrochemical Science Advances: e2100207.
[9] Pindoo, Irfan Ahmad, and Sanjeet K. Sinha. "Increased sensitivity of biosensors using evolutionary algorithm for biomedical applications." Radioelectronics and Communications Systems 63.6 (2020): 308-318.
[10] Akbari jonous, Zahra, et al. "An electrochemical biosensor for prostate cancer biomarker detection using graphene oxide–gold nanostructures." Engineering in Life Sciences 19.3 (2019): 206-216.
[11] Crulhas, Bruno P., et al. "Electrochemical aptamer-based biosensor developed to monitor PSA and VEGF released by prostate cancer cells." Analytical and bioanalytical chemistry 409.29 (2017): 6771-6780.
[12] Arneth, Borros M. "Clinical significance of measuring prostate-specific antigen." Laboratory Medicine 40.8 (2009): 487-491.
[13] Abdolkader, Tarek M., et al. "ISFET pH-sensor sensitivity extraction using conventional MOSFET simulation tools." International Journal of Chemical Engineering and Applications 6.5 (2015): 346.
[14] Sant, William, et al. "On-line monitoring of urea using enzymatic field-effect transistors." Sensors and Actuators B: Chemical 160.1 (2011): 59-64.
[15] Cazalé, Arnaud, et al. "Study of field-effect transistors for the sodium ion detection using fluoropolysiloxane-based sensitive layers." Sensors and Actuators B: Chemical 177 (2013): 515-521.
[16] Sinha, Soumendu, et al. "Fabrication, characterization and electrochemical simulation of AlN-gate ISFET pH sensor." Journal of Materials Science: Materials in Electronics 30.7 (2019): 7163-7174.
[17] Dutta, Jiten Ch. "Ion sensitive field-effect transistor for applications in bioelectronic sensors: A research review." 2012 2nd National Conference on Computational Intelligence and Signal Processing (CISP). IEEE, 2012
[18] Muangsuwan, Wannaporn, et al. "Development of an immunoFET biosensor for the detection of biotinylated PCR product." Heliyon 2.10 (2016): e00188.
[19] . Madec, Morgan, et al. "Environment for modeling and simulation of biosystems, biosensors, and lab-on-chips." IEEE Transactions on Electron Devices 66.1 (2018): 34-43.
[20] Jiang, Yuting, et al. "Transient electroosmotic slip flow of fractional Oldroyd-B fluids." Microfluidics and Nanofluidics 21.1 (2017): 7.
[21] Bazant, Martin Z., and Todd M. Squires. "Induced-charge electrokinetic phenomena: theory and microfluidic applications." Physical Review Letters 92.6 (2004): 066101.
[22] Hu, Guoqing, Yali Gao, and Dongqing Li. "Modeling micropatterned antigen-antibody binding kinetics in a microfluidic chip." Biosensors and Bioelectronics 22.7 (2007): 1403-1409.